Fig. 3

Concentration-dependent effect of uridine on the induction of long-term potentiation. Brain slices are treated with uridine and fEPSPs are recorded for at least 15 min (baseline period). Then, HFS is delivered and fEPSPs are collected for an additional 45 min. Post-tetanic potentiation (PTP) is measured 1 min post-HFS, short-term potentiation (STP) is calculated 10–15 min post-HFS, and long-term potentiation (LTP) is measured 40–45 min post-HFS. a Left, graph showing the normalized fEPSP slopes (mean ± SEM) for the uridine (3 μM) and control groups; the arrow marks HFS. Right, bar graphs show that uridine (3 μM) does not significantly affect any plasticity time point. b Uridine (30 μM) has a lowering effect on STP and LTP, but PTP is unchanged; * P < 0.05 (t-test). c Uridine (300 μM) significantly decreases PTP, STP, and LTP; * P < 0.05 (t-test). d Uridine (300 μM) has a null effect on LTP expression, when introduced 10 min post-HFS, following the induction of LTP. e Graphs showing the negligible effect of uridine (30 μM, 300 μM) on LTP expression. Numbers within bars indicate number of brain slices per group