Fig. 5

Protective effect of uridine against oxygen-glucose deprivation. a Graph showing normalized fEPSP amplitudes (mean ± SEM) for brain slices that are treated in uridine (100 μM) for 30 min before receiving an oxygen-glucose deprivation (OGD) insult (6 min). b In this set, brain slices are in uridine (100 μM) for 45 min before receiving an OGD insult (6 min). c Plot showing the percent amplitude deficit for the 45 min uridine group compared to the untreated control group. In the uridine group, the amplitude deficit disappears by 45 min post-OGD (vs. 65 min in control), highlighting the protective action of uridine. d Graph showing the total OGD deficit, which is calculated from the percent amplitude deficit plots by taking the area-under-curve (AUC) for three different incubation periods: 15 min, 30 min, and 45 min. The short incubation (15 min) is insufficient for a protective effect against 6 min OGD, while the longer incubation periods (30 min and 45 min) significantly reduce the magnitude of the OGD-induced deficit; *, P < 0.05; **, P < 0.01 (t-test). Numbers within bars indicate number of brain slices per group