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Fig. 2 | Bioelectronic Medicine

Fig. 2

From: The role of nicotinic receptors in SARS-CoV-2 receptor ACE2 expression in intestinal epithelia

Fig. 2

Single-cell RNA-sequencing analysis of ileal epithelial cells. Data were obtained from GSE134809 (Martin et al. 2019). a Uniform manifold approximation and projection (UMAP) visualization of the unsupervised clustering analysis of all cells. b Heatmap of the percentage cells in a cluster found to be non-zero for epithelial cell markers VIL1 and CDH1 as well as SARS-CoV-2-associated genes ACE2 and TMPRSS2. c Visualization of the expression of the aforementioned genes on the UMAP. d UMAP visualization of the unsupervised clustering analysis of the epithelial cells. e Heatmap of the percentage epithelial cells in a cluster found to be non-zero for markers of stem cells (LGR5, ASCL2, OLFM4, GKN3, SLC12A2, AXIN2), goblet cells (MUC2, TFF3, CLCA3, AGR2), enterocytes (FABP1, ALPI, APOA1, APOA4), enteroendocrine cells (CHGA, CHGB, TAC1, TPH1, NEUROG3), and tuft cells (DCLK1, TRPM5, GFI1B, IL25). f Visualization of the normalized counts for the nAChR genes CHRNA5, CHRNA7, CHRNA10, CHRNB1, and CHRNE on the x-axis against ACE2 or TMPRSS2 on the y-axis for all epithelial cells, where each dot represents an individual cell (Martin et al. 2019)

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