Fig. 2

Experimental setup. A) 3D model of the experimental setup. The black box at the bottom is the MEA headstage, at the top of which the custom-made lid is placed. The circular holes in the lid are aligned to the underlying MEA plate. The ultrasound transducer is inserted in the transducer holder structure which can then be positioned in the engraved alignment holes for precise centering with respect to the MEA well. B) Cross-section view of the experimental setup showing the relative position of the transducer with respect to the neuronal culture. C) Detail picture of the experimental setup showing the transducer in the support structure on the left and on the right, the wells with the coloured cap are used to record the trigger signal. D) Schematic view of the complete experimental setup showing the connections between the various elements