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Fig. 2 | Bioelectronic Medicine

Fig. 2

From: Anatomical differences in nociceptor neurons sensitivity

Fig. 2

JNC neurons are more sensitive to TRPV1, TRPA1 and TRPM8 stimulation. Naïve C57BL/6 mouse JNC and DRG neurons were harvested, cultured (2,500 neurons/plate) for 24h, loaded with a calcium responsive dye (Fura-2AM), and sequentially exposed to the TRPV1 agonist capsaicin (300nM; A-B), the TRPA1 agonist menthol (100uM; C-D), or the TRPA1 agonist JT010 (1uM, E-F) followed by KCl (40mM; positive control; A-F). The number of responsive (A, C, E) cells to capsaicin, menthol, and JT010 is increased in JNC neurons. The average amplitude of responses are shown in (B, D, F). Data are shown as mean ± S.E.M. Number of dishes tested is shown. N are as follows: A-C-E: n=5-6 dishes/group, B-D-F: n=290-1200 neurons/group. P-values are shown in the figure and determined by unpaired Student's t-test (A, C, E)

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