Fig. 1

Elevated peritonitis-induced liver leukocyte content in vagotomized mice. Wild-type C57BL/6 J mice were subjected to either left cervical unilateral vagotomy (VX, grey bars) or sham surgery (SHAM, white bars). After 7 days, mice were injected intraperitoneally with zymosan (0.1 mg/mouse) and euthanized 12 h thereafter. Liver homogenates were analyzed by flow cytometry for (A) CD45⁺, (B) CD45⁺CD11b⁺F4/80⁺, (C) CD45⁺CD11b⁺F4/80⁺Tim4⁻, (D) CD45⁺CD11b⁺F4/80⁺Tim4⁺MHCII⁺, and (E) CD45⁺CD11b⁺F4/80⁻Ly6C⁺ cell subsets. Results were normalized to liver biopsy mass (g). n = 6–7 per group. Results are expressed as mean ± SEM. ns = not significant; * p < 0.05; ** p < 0.01 (two-sided Student´s t-test; Welch’s correction was applied as appropriate)