Fig. 2

Characterization of PCL puro encapsulated microspheres. A SEM image demonstrating the size and morphology of the lyophilized microspheres. An accelerated voltage of 1.0 kV and working distance of 9.2 mm was used to acquire the image. Scale bar is 10.0 µm. B Histogram of puro microsphere diameters with a sample size of n = 90. Phase contrast images of C AD hiPSCs, D AD NPCs, E 2D cultured BFCN’s on day 10 and F day 20 of culture. Bright phase images of constructs with cells (G) and cells and microspheres (H). Scale bar is 100 µm