Figure 3
From: Neuronal Circuits Modulate Antigen Flow Through Lymph Nodes
Antigen flow restriction is dependent on sensory neural input. (A, B) Mice immunized with KLH were injected with bupivacaine at the femoral and sciatic nerves. Upon challenge with KLH-800CW, an increase in antigen signal was seen in the sciatic lymph node. (A) Images are representative; triangles indicate popliteal lymph nodes and stars represent sciatic lymph nodes. (B) Antigen signal in the sciatic lymph node in individual animals is shown here. (mean ± SEM: saline, 31.45 ± 3.759, n = 10, bupivacaine, 45.65 ± 5.350, n = 9, p < .05 by t test). (C, D) NaV1.8-DTA mice were immunized with KLH. Images are representative; triangles indicate popliteal lymph nodes and stars represent sciatic lymph nodes. (D) Antigen signal in the sciatic lymph node in individual animals is shown here. (mean ± SEM control, 15.41 ± 3.526, n = 13; Nav1.8-DTA, 35.56 ± 6.035, n = 16, p < .05 by t test). (E, F) No difference in the circulating levels of (E) IgM and (F) IgG was seen in NaV1.8-DTA mice (IgM, NaV1.8-depleted, 0.5044 ± 0.05477, n = 9 versus control, 0.5466 ± 0.08038, n = 6) (IgG, NaV1.8-depleted, 0.7737 ± 0.09626, n = 9 versus control, 0.6340 ± 0.2365, n = 5). (G) Immunized TRPV1-DTA, NaV1.8-DTA and littermate control mice were injected with KLH-800CW in the hind paw. The increase in antigen seen in NaV1.8-DTA mice was not recapitulated in TRPV1-DTA mice in the sciatic lymph node (control, 6.783 ± 1.290, n = 9; TRPV1-DTA, 6.345 ± 1.547, n = 10; NaV1.8-DTA, 43.93 ± 15.57, n = 6, p < .001 by one-way ANOVA Bonferroni post-test). Data represent individual values and mean ± SEM.