Antigen flow restriction is dependent on sensory neural input. (A, B) Mice immunized with KLH were injected with bupivacaine at the femoral and sciatic nerves. Upon challenge with KLH-800CW, an increase in antigen signal was seen in the sciatic lymph node. (A) Images are representative; triangles indicate popliteal lymph nodes and stars represent sciatic lymph nodes. (B) Antigen signal in the sciatic lymph node in individual animals is shown here. (mean ± SEM: saline, 31.45 ± 3.759, n = 10, bupivacaine, 45.65 ± 5.350, n = 9, p < .05 by t test). (C, D) NaV1.8-DTA mice were immunized with KLH. Images are representative; triangles indicate popliteal lymph nodes and stars represent sciatic lymph nodes. (D) Antigen signal in the sciatic lymph node in individual animals is shown here. (mean ± SEM control, 15.41 ± 3.526, n = 13; Nav1.8-DTA, 35.56 ± 6.035, n = 16, p < .05 by t test). (E, F) No difference in the circulating levels of (E) IgM and (F) IgG was seen in NaV1.8-DTA mice (IgM, NaV1.8-depleted, 0.5044 ± 0.05477, n = 9 versus control, 0.5466 ± 0.08038, n = 6) (IgG, NaV1.8-depleted, 0.7737 ± 0.09626, n = 9 versus control, 0.6340 ± 0.2365, n = 5). (G) Immunized TRPV1-DTA, NaV1.8-DTA and littermate control mice were injected with KLH-800CW in the hind paw. The increase in antigen seen in NaV1.8-DTA mice was not recapitulated in TRPV1-DTA mice in the sciatic lymph node (control, 6.783 ± 1.290, n = 9; TRPV1-DTA, 6.345 ± 1.547, n = 10; NaV1.8-DTA, 43.93 ± 15.57, n = 6, p < .001 by one-way ANOVA Bonferroni post-test). Data represent individual values and mean ± SEM.