Figure 4
From: Neuronal Circuits Modulate Antigen Flow Through Lymph Nodes
Stimulation of neuronal activity restricts antigen flow. (A, B) Electric stimulation of the nerves to the popliteal and sciatic lymph node restricts KLH-800CW flow to the sciatic lymph nodes (sham, 114.1 ± 16.17, n = 5 versus electrically stimulated, 56.76 ± 14.38, n = 5, p < .05 by t test). (A) Images are representative. (B) Antigen signal in the sciatic lymph node in individual animals is shown here. (C, D) A noninvasive magnetic field was applied to the hind legs of naïve mice to induce nerve activity, followed by injection of KLH-800CW. In magnetically stimulated animals, lower levels of antigen were observed in the sciatic lymph nodes (sham, 55.99 ± 4.993, n = 10 versus magnetically stimulated, 30.87 ± 4.169, n = 12, p < .001 by t test). (C) Images are representative; triangles indicate popliteal lymph nodes and stars represent sciatic lymph nodes. (D) Antigen signal in the sciatic lymph node in individual animals is shown here.