Figure 5

Time course for TNF- and IL-1β-mediated neurograms. (A) (Top) Trace showing vagus nerve activity under vehicle (200 µL sterile saline, marked by line), which is used as control. (Bottom) Graph describing the frequency of CAP firing (mean ± SEM, gray area) for N = 6 mice starting 10 min prior to saline injection. (B) (Top) Representative neurogram for TNF (dose of 50 µg, marked by line). (Bottom) Graph showing the mean frequency ± SEM (blue area) for N = 21 mice. (C) (Top) Representative neurogram for IL-1β (dose of 350 ng, marked by line). (Bottom) Graph showing mean frequency ± SEM (green area) for N = 10 mice. (D) Plot depicting the total CAP counts (mean ± SEM) in the 10 min immediately after TNF administration at the indicated doses (N = 5–7 mice per dose). (E) Plot showing total CAP counts in the 5 min following IL-1β treatment at the indicated doses (N = 5–9 mice per dose).