Fig. 4From: Standardization of methods to record Vagus nerve activity in miceThe effect of isoflurane on the baseline vagus nerve activity. Baseline vagus nerve activity was recorded for 30 min at different levels of isoflurane, 1.5% (n = 5), 1.75% (n = 5) and 2.0% (n = 4), using the cuff electrode. a Spikes rate of the neural events recorded for each isoflurane level. A significant difference in the baseline activity was found over three different levels of isoflurane (two-way ANOVA, p < 0.0001). b Total spike count over the entire 30-min recording. Total spikes with 1.5% isoflurane are significantly higher than 1.75% (Mann-Whitney, p < 0.05, U = 2) and 2% levels (Mann-Whitney, p < 0.05, U = 1). c Total spikes in the final 10 min of the recording. Total spikes with 1.5% isoflurane are significantly higher than 1.75% (Mann-Whitney, p < 0.01, U = 0) and 2% levels (Mann-Whitney, p <0 .05, U = 0). d The variance of the spike rate for each individual mouse over the 30-min recording period. Each dot represents variance in each mouseBack to article page