Fig. 1

Lumbar and vagal neurons transcriptomes are different. (A-C) The whole jugular nodose ganglia complex (JNC; n=3; red) and dorsal root ganglia (DRG; n=3; blue) mouse transcriptomes were profiled using RNA sequencing (RNA-seq; Illumina HiSeq 2500) by Kaelberer et al., 2020. In-silico analysis of the neuron's transcriptome revealed that naïve mouse JNC nociceptor neurons express different neuropeptides (A), ion channel receptors (B), and growth factor receptors (C) than the one found in DRG. JNC neurons are enriched for Vip, Trpv1, Trpa1, and Ntrk2, while DRG neurons show higher higher levels of Calca, Tac1, Trpm8, Trpm3, Piezo2, Gfra1 and Ntrk1. (D) Gene expression was also assessed by RT-qPCR after 24 hours of culture (D). ΔΔCt was calculated in comparison to JNC. Trpm8 and Calca were enriched in DRG neurons, while Trpa1 was enriched in JNC neurons (D). Expression across datasets is shown as Fragments Per Kilobase of transcript per Million reads mapped (FPKM) (A-C) or ΔΔCt with JNC as the control condition (D). Data are shown as means ± S.E.M. N=3/groups. P-values are shown in the figure and determined by unpaired multiple Student t-test with Hidom-Sidak corrections (A-D). Experimental details were defined in Kaelberer et al., 2020 (A-C)