Fig. 3

JNC neurons are insensitive to NGF. Naïve C57BL/6 mouse JNC and DRG neurons were harvested, cultured for 24h in the presence of Nerve Growth Factor (NGF, 50 ng/mL). The cells were then loaded with a calcium responsive dye (Fura-2AM) and sequentially exposed to capsaicin (100 nM) followed by KCl (40mM; positive control; A). The addition of NGF increased the number of capsaicin-responsive neurons in DRG cultures but not in the one from the JNC (A). Similarly, NGF increased capsaicin-induced CGRP release from cultured DRG neurons (B). Data are shown as mean ± S.E.M. Number of dishes tested is shown. N are as follows: A: n=5-10 dishes/group from two independent experiments, B: n=6 wells from different animals/group from two independent experiments. P-values are shown in the figure and determined by unpaired Student's t-test (A) or one-way ANOVA post hoc Dunnett (B)