Fig. 2
From: Hepatic stellate cell activation markers are regulated by the vagus nerve in systemic inflammation
Elevation of peritonitis-induced pro-inflammatory mediators in liver homogenates and blood from vagotomized mice. Wild-type C57BL/6J mice were subjected to either left cervical vagotomy (VX, grey bars) or sham surgery (SHAM, white bars). After 7 days, mice were injected intraperitoneally with zymosan (0.1 mg/mouse) and euthanized after 12 h. Liver and blood samples were collected and analyzed by qPCR and ELISA. (A-C) Transcripts of pro-inflammatory mediators in liver homogenates were measured by qPCR. Bars show mean ± SEM, dots represent individual mice. Transcript levels were normalized to Ppia and expressed relative to SHAM (100%). (D) Plasma CCL2 was measured by ELISA. n = 7 mice per group. Bars show mean ± SEM, dots represent individual mice. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. (two-sided Student´s t-test)