Figure 7

Genetic ablation of cytokine-mediated neurograms. (A, B) Plots showing the total CAP count in the 5-min period immediately after IL-1β or TNF administration in KO mice. (A) In TNFR1/2KO mice, responses for IL-1β (N = 8) and TNF (N = 5) are significantly different (*, P < .05, MW test). (B) In IL1RKO mice, CAPs for IL-1β (N = 8) and TNF (N = 8) are also statistically significant (*, P < .05, MW test). (C) Cultured nodose ganglia are stained for NeuN (left panel) to identify all cultured neurons, antibody for TNFR1 (green panel) and antibody for IL1R (red panel); merged signals are shown in the right panel. Scale bar, 50 µm. (D) Responsive neurons display a ≥ two-fold increase in Fluo-4 fluorescence in response to 100 ng/mL cytokine. (Top) Plot of neurons that respond to TNF in WT (N = 7) and TNFR1/2KO (N = 7) mice. (Bottom) Neurons that respond to IL-1β in WT (N = 5) and IL1RKO (N = 4) groups; *, P < .05, MW test.